FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2354711
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10050.039.9484521
TCTGTCG9900.036.6157158
TCGCTCT10650.034.03574
GTCTTGC21400.030.0173421
TCTCGCT13000.028.9990332
TCCGGTA1050.00771179327.6181262
GTACAAT6100.027.5233521
GTTAGCC10900.027.2691523
CTGTCGC13900.027.1219739
TAATACT7500.026.0985764
TTGCTCT29550.025.7600124
GTATATA8000.025.5488531
CGCTCTG13550.025.1478315
GTACGGA2051.2246723E-524.925711
CTCGCTC13400.024.8867023
GTGTTAG13800.024.8612021
GTCTCAC26850.024.7400441
GTCTTAC9100.024.0648521
GTGTAAA13300.023.6004411
GTATTGG7450.023.515721
Produced by FastQC (version 0.11.5)