FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347163

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347163
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247547
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7950.045.597448
GTCGTAC800.002013912436.334841
TCGGTAC1051.8517966E-434.5322233
GTCTCGC9750.032.7945251
TCGCTCT10900.031.9343854
GTATTAC4100.031.9037631
CTGTCGC12000.030.8131549
ACCGTTA1000.00607607928.999978
TAAACCG1255.157238E-428.9986785
GTATTAT5650.028.296161
GTGTAAA10800.028.2604311
TATACTG5150.028.1540575
GTATAAC2853.1468517E-1028.0479491
TCTCGCT11300.026.958272
CGCTCTG12000.026.5821235
GTATCGT1100.00956449526.4253391
GTCTTGC19350.026.2887741
TAGTACT4900.025.1591914
CTCGCTC11850.024.4785393
GTCTCAC23300.024.3271881
Produced by FastQC (version 0.11.5)