FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2247547
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7750.044.2667731
TCTGTCG7450.041.8434228
GTATAAG5500.038.487261
TCGCTCT10150.033.5689054
GTCTTGC17200.032.677131
ACTGTCG2401.5843398E-930.2067348
TCTCGCT12500.027.8403852
CTCGCTC11200.027.1854573
CTGTCGC12500.026.6785879
GTTTCGC3251.375156E-924.705351
TTGCTCT26500.024.620794
GTATTGT5100.024.3310261
GGTATAC3001.5907062E-824.3310261
GTATAGG3652.0190782E-1023.9977231
CGCTCTG11800.023.3457035
GTCTCAC20400.022.899791
TACGGAA1902.1941472E-422.8950522
CTTGCTC25800.022.478933
GGAGTGC44300.022.4193085
TCTGTCA22550.021.861378
Produced by FastQC (version 0.11.5)