FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347165

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347165
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320218
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8800.038.720698
GTATAAT6400.034.049631
GTCTCGC12000.033.8982961
GTCTTGC22550.032.5346341
TCGCTCT12050.031.8982264
GTACCGT1204.0143103E-430.2663361
CGCTCTG13050.028.3320665
CTGTCGC12300.028.2926399
GTATAGT5200.027.9381541
TCTCGCT14400.027.2009372
GTATTAT7000.026.9802761
GTGTAAA14050.026.884261
TACACTG12750.026.1556935
GTATTAG6950.026.1292111
GTATAAG6750.025.8272741
CTCGCTC14650.025.2470263
GTATTAA6400.024.9697281
GTGTTAG15500.024.8379231
GTGTTAC9950.024.8214361
GTATAGG5600.024.6454431
Produced by FastQC (version 0.11.5)