FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347166

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347166
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2320218
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8650.051.9639638
GTCTCGC10150.043.8681141
TCGCTCT12100.036.5454864
GTCTTGC18150.036.1952061
CTGTCGC12700.034.251089
GTATTAA6750.033.5230141
CGCTCTG13050.031.6651615
CTCGCTC13350.030.9535853
TCTCGCT13850.030.3615322
GTATAAG4850.028.5954571
TACGTAC1306.4873527E-427.883293
GTGTTAG13150.026.088991
GTCTCAC26200.025.9099661
GTGTAAC5950.025.762471
GTATTAT6100.025.1289671
GTTTCAC33150.024.4935553
GTGTAAT8150.024.1819521
GTATAGG5800.023.9117181
GGGTTTC36200.023.7935141
GTACGGA1851.7493305E-423.673621
Produced by FastQC (version 0.11.5)