FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347168

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347168
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1978856
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7600.052.463978
GTCTCGC8150.045.6700361
CTGTCGC10200.041.2230229
GTCTTGC19050.036.778591
GCGTAAC800.00197183336.4912571
TCGCTCT10150.036.4254724
CTCGCTC11350.033.213873
TCTCGCT11350.032.5784382
GTATAGG4950.030.9622781
TAGACCG1204.060889E-430.2065285
GTATAAT6050.030.1580621
GTATTAG6500.029.1930071
TAATACG1255.1582075E-428.9975344
GTCTTAC8150.028.655711
CGCTCTG11500.027.737475
GTACTAA3750.027.2468051
CGACGAA1100.00967307426.364058145
GTATTAT6400.026.228091
GTGTTAG13050.025.7256361
TTACACT10150.025.71214
Produced by FastQC (version 0.11.5)