FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347169

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347169
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1890397
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7450.041.8457378
CGACGAA700.001057005441.427574145
GTATAAC3350.032.529021
GTCTCGC7700.032.07841
TATAACG1153.1542906E-431.5368292
GTCTTGC17050.031.1044831
GTATAAT5850.031.0462131
GTGTAAC4950.030.8204231
CTGTCGC10350.030.1208449
CCGTAGT1255.094326E-429.0592561
CTTACCG1504.400684E-529.0000699
TCGCTCT8900.028.521114
GTGTTAC8100.026.906721
GTCTTAC7100.026.6035441
GTACTAT3950.025.7487071
GTATTAT5750.025.2689171
TCTCGCT9700.024.6767562
GTATAAG5600.024.6484761
GGTATAC3601.8007995E-1024.2160471
GGGTTTC31550.024.177671
Produced by FastQC (version 0.11.5)