FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347170

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347170
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1890397
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7700.046.1346978
GTCTCGC9050.039.518241
GTCTTGC15500.039.083781
TCGCTCT9600.037.7570534
CGCCGTT1656.022128E-835.151173145
CGCTCTG10350.032.9206435
CTCGCTC11050.030.8359893
CTGTCGC11400.029.8892699
TCTCGCT12000.029.6054482
GTATAAG5950.029.4404491
GAGTTAC5800.026.4266091
GGGTTTC30150.026.3869551
TAGTCCG1400.001000154225.8912375
GTATTGG6550.025.6292971
ACCATGT23500.025.605468
GTTTCAC27250.025.5403613
GGGTACA8450.025.049061
GGTCTAC4201.8189894E-1224.329261
GTATTAC4550.024.0619051
GTATAGC3952.1827873E-1124.0212941
Produced by FastQC (version 0.11.5)