FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347171

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347171
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1880760
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7350.040.441488
ATACCGG951.0293408E-438.1572386
GTCTTGC16150.037.335851
GTCTCGC7800.036.3237341
TCGCTCT7400.035.2809144
GTGTAAG8300.032.3850141
GTATTAG4700.030.9138161
CTGTCGC10600.029.409879
GCGCATA1000.00601577929.0589851
GTTTCAC26800.028.1428223
CTCGCTC9100.027.0960923
GTATAAT5900.027.0888861
TCTCGCT9950.026.9715252
GTCTTAC7900.026.6680581
GTATTAT5750.026.532121
GTACTAG3056.9485395E-1026.2007261
GGGTTTC29950.025.9541871
TTACACT9300.025.7335724
GATCGTT2001.0662623E-525.3745619
GGATAGG7300.024.8792691
Produced by FastQC (version 0.11.5)