FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347172

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347172
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1880760
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8050.051.70591
TCTGTCG8900.046.434448
TCGCTCT8750.041.424724
CTCGCTC9650.038.3135223
TCTCGCT10800.035.582022
GTCTTGC16550.035.298251
CTGTCGC11750.033.320489
CGCTCTG10450.033.301885
GTATACG1000.00586601929.2092971
GGGTTTC27000.028.9388391
GTTTCAC24200.028.758423
GTCTTAC7100.027.7694031
GTCTCAC20450.026.4240591
GTGTTAC6700.026.1575811
TTGCTCT21250.025.9270024
GTACTAG3159.531504E-1025.500181
GTATTAA4350.025.180431
TCTGTCA22200.025.1474028
CTTGCTC22250.024.7624023
GTGTAAG9200.024.605661
Produced by FastQC (version 0.11.5)