FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347173

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347173
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896429
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7500.049.2994968
GTCTCGC6500.043.5730861
CTGTCGC9950.037.889069
TCGCTCT8000.034.447144
TCTCGCT8950.031.6051882
GTATAGA4850.029.9471341
GTATTAC3650.029.844581
CTCGCTC9250.029.7913343
GTCTTGC18250.029.0487211
GTATTAA5900.028.3101941
ATTATAC5900.027.0407523
GTATTAG5700.026.7554041
AATACCG1100.00967420726.3633695
GTGTAAA9150.026.191471
CTCGATC7800.026.0253758
GTGTTAG11350.025.593591
GTACTAG3451.05501385E-1025.2597581
TCGATCT7750.025.2578079
GAACCGT1450.001226921624.9997466
CGCTCTG10500.024.8568885
Produced by FastQC (version 0.11.5)