FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1896429
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG300.00193601972.499929
TCTGTCG8000.041.6874548
GTCGTAG1105.0375274E-639.8025441
GTCTCGC8850.037.92861
TCGCTCT9500.034.339354
GTCTTGC16250.033.6790731
TATACCG900.00362906532.2221875
ATACCGT900.00362906532.2221876
TCTCGCT10850.031.4046692
CTGTCGC10450.031.2200629
GTACTAG3201.8189894E-1229.6446021
GTACTAT3700.029.582971
CTCGCTC10900.029.264483
GTATAAA6550.027.851651
AACGGCG1050.007710207327.6190175
CGCTCTG10800.027.5231175
GTCTCAC20550.027.3420521
GTACGGG1658.059267E-526.5350281
GTGTTAG12650.025.958181
GTATAGG3950.025.8632551
Produced by FastQC (version 0.11.5)