FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2206475
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCTCT10800.038.953354
TCTGTCG9900.038.814528
GTCTCGC10000.038.520661
CGCTCTG11700.034.0793275
TCTCGCT11750.033.9581642
GTATTAC5450.032.006091
CTCGCTC12850.030.481143
CTGTCGC13150.030.3249669
GTACTAG4200.029.4182931
GTCTTGC21200.029.1407621
GTATTAG6700.028.2043691
GTGCGTA2104.88375E-727.6878051
GTATAGA5950.026.8734571
GTGTAGT12250.026.6989541
GGTATAC4100.026.5904221
GTATAAT6750.025.8419511
GTACTAA4500.025.841951
GTGTTAT7050.025.7732221
GTATTAT4950.024.9609761
GTGTTAG15500.024.3831311
Produced by FastQC (version 0.11.5)