FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2206475
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9750.035.6908348
GTCTCGC9250.033.148941
GTCTTGC18450.032.4473761
CTGTCGC11900.029.242499
TACGACA1255.156393E-428.999462
GTATTAG7150.027.5689241
GTACTAG3850.026.5478531
GTGTTAG13850.025.3019241
GTCTCAC24150.023.579771
GTGTAAA12400.023.5505121
TCGCCGG5000.023.200619145
GTATAAT5700.023.0547161
TCGCTCT11350.022.9936054
GTGCTAT8600.022.9206751
GTTAGCC12400.022.8014773
GTGTAAG11600.022.6572191
GTAGACG2252.6542622E-522.5546253
GCGTATG1650.002498740822.123211
CTTAGCG2303.1452415E-522.0643069
TAACACT10850.022.0487824
Produced by FastQC (version 0.11.5)