FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2073483
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8300.046.6169471
TCTGTCG6950.043.8100058
GTCTTGC18800.037.6669041
TCGCTCT10300.035.895654
TCTCGCT11000.032.294842
TACACCG1601.879389E-631.7166185
CTCGCTC11750.030.8489933
GTATAGG6200.030.6145361
GTGTAAC5800.030.208511
CTGTCGC10550.029.5477779
GTGTTAG12400.028.2595731
GTCTCAC20950.028.2258511
CGCTCTG12250.027.8144595
GTGTAAT8250.027.4317681
GTCTTAC7250.027.1876581
CCGGTCG1358.08883E-426.8500489
GTATTAG6000.026.7680971
GTGTAAA12950.026.4956231
TTGCTCT26000.026.4886044
TCTGTCA25850.026.0814198
Produced by FastQC (version 0.11.5)