FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1821078
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6600.036.2507558
TCTATCG800.002036845336.2507558
GTATAGG4550.035.1289831
GTCTTGC15700.032.8558731
GTCTCGC7600.030.5907881
GTACTAC3950.029.4291131
GTACCGT1255.092205E-429.061251
TTATACT4250.029.0077744
GTGTTAG12700.028.0315231
TCGCGCA1606.830199E-527.1880659
TCTCGCT9100.027.0974042
GTATTAG5650.027.0038151
GTAACGA1100.00966229626.3699783
GTCTAGC6350.026.3153061
TCGCTCT10200.026.3060684
TATACTG5250.026.237925
GTATTAT4900.025.2061841
CTGTCGC8750.024.857669
GTATTAA5000.024.7020651
GGAGTGC42100.024.2813135
Produced by FastQC (version 0.11.5)