FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347180

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347180
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1821078
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATACG1402.5465852E-1046.92311
CGCGAAA657.338949E-444.61443145
GTCTCGC8050.042.616151
TCTGTCG8200.042.4392858
CGCTCTG9100.033.4608235
TCGCTCT9400.033.162364
GTCTTGC16450.032.835081
CTGTCGC11650.031.7383929
CTCGCTC9300.031.1804143
GTCTCAC20250.029.9174981
GTCTTAC6650.028.5380251
TACACCG2104.9911796E-727.6184565
GTACAGT9600.026.6114791
TCTCGCT11450.026.5925872
GTACGGA1709.854409E-525.7617021
GTATTAT4950.025.0677831
GTGTAAA11400.024.9707721
TCGCCGG4400.024.715382145
TTGCTCT21550.024.5572914
TGTACGG1500.001495168524.1654852
Produced by FastQC (version 0.11.5)