FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1991095
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8400.037.1122868
TCGCTCT10150.033.5791244
CTGTCGC10600.030.777639
GTCTCGC9300.029.6848851
GTCTTGC17650.029.2245861
GTGTAAC5100.025.6411231
GTACAGA10350.025.269511
GTGTATA7500.025.1852821
GTTTCGC4051.8189894E-1225.1135271
TCTCGCT11100.024.8292542
GTGTTAA8600.024.4982051
TACACGG2101.55664E-524.1655335
CGCTCTG11250.023.8433255
TTTCGCT4002.7284841E-1123.5714572
GTGTTAG12700.023.4538881
GTGTAGA9050.022.4773031
GTGTAGC7150.022.35381
GTGTAAG8800.022.2902951
GTACTGA6200.022.2636641
GTCTAGC7850.022.211421
Produced by FastQC (version 0.11.5)