FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931105
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7750.052.3871358
GTCTTGC16350.037.3173031
TTAAGCG1102.4317934E-432.966524
GTGTTAC8000.030.8700751
CTGTCGC12050.030.684679
ACCGTGT6850.029.6350578
GTCTCGC8500.029.0541881
TCGCTCT8850.028.6827374
GTACTGA6800.026.7042161
TCGATCT7900.026.6139419
TTCACCG6100.026.1462065
GCGTTAT1409.997931E-425.8928766
CTCGATC7650.025.5882518
GTATTAG6250.025.5676821
GTATAAG4550.025.5421431
GAGTGCA49750.025.35686
GTTTCAC28350.025.3266583
TCTCGAT7750.025.2580837
GGAGTGC46300.025.0527175
CCGTGTT7250.025.0000179
Produced by FastQC (version 0.11.5)