FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347188

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347188
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1931105
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7150.045.931311
TCTGTCG8550.043.244348
TCGCTCT8050.042.3246544
GGTATAC3850.036.015981
GTCTTGC15950.034.7740481
CGCTCTG9350.033.34045
GTACGGG1352.0487101E-532.435441
GTGTAGT9300.032.1738661
TCTCGCT10250.031.828332
CTGTCGC11950.031.5471889
GTGCGTA1402.6269034E-531.2770371
TAATACT4750.030.5230454
GTACGGA1952.462948E-729.9404071
GTATAAA5750.029.1918981
CTCGCTC10500.028.9983943
GTGTAAA10600.027.5395261
GTATTAA4100.026.6999071
CTATCCG1100.00967515926.3628629
GTGTTAC6700.026.1419981
GTCTCAC21650.025.9558471
Produced by FastQC (version 0.11.5)