FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2123050
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8150.047.145628
GTCTCGC8900.037.555531
TCGCTCT9800.037.0059554
CTGTCGC11500.033.4118929
CGTTTCG1751.0104122E-733.1417169
TACGCTA900.003629754332.221115
CGCTCTG10300.031.6736665
GTCTTGC20850.031.3648011
TTGTACG1000.006063023629.0126674
GTCTTAC8400.028.5457021
GTATAGG5350.028.521451
TCTCGCT11450.027.8776862
ATACCCG2104.97188E-727.6311134
GGTATAC3700.027.4936491
TTGCTCT26500.026.823034
CTCGCTC11800.026.431033
GTATAAT6200.025.7832151
GTGTTAC8500.025.6453361
GTGTAAT8300.025.3878541
GTGTAGG12450.024.512411
Produced by FastQC (version 0.11.5)