FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347191

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347191
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2376544
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG9250.046.2414258
TCGCTCT11100.035.9333534
GTCTCGC12350.035.305221
CTGTCGC12800.034.5494239
GTACGTT1102.4332038E-432.963653
GTCTTGC22000.032.7014661
TCTCGCT12650.030.3915652
CGCTCTG12950.030.230475
GTGTTAC11100.030.1154631
GTATTAT6500.029.0679661
CTCGCTC13450.028.0374873
GTCTTAC9900.027.1594641
TAATACT7000.026.9360124
GTATTAG6950.026.1402591
GTGTTAG15400.025.481661
TGTACCG2001.066599E-525.3740025
GGTATAC4400.024.7738341
GTGTAAA14450.024.6423951
GTATAAG6250.024.4170931
TACACTG14300.024.3347075
Produced by FastQC (version 0.11.5)