FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347193

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347193
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2280936
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9350.042.7435151
GTCTTGC18650.035.8449631
TCTGTCG8600.035.4061558
TCGCTCT10350.035.034094
TCTCGCT10950.034.444282
GTACGAG1153.1217234E-431.5930331
GTATTAT6550.029.9530891
GTATAGG5750.029.065591
CTCGCTC12300.028.8903083
CGCTCTG12400.028.0632485
GTATTAA6500.027.9476811
CTGTCGC11350.027.466329
GTATAAT6350.027.463551
GTGTTAA8200.026.5843831
GTACTAG4400.026.4232621
GGTCTAC5250.026.2974381
GTGTAGG10550.026.1728061
GTATTAG7550.025.985791
GCGAATA1450.001210872225.0565431
GTTTACG1450.001210872225.0565431
Produced by FastQC (version 0.11.5)