FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2280936
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10200.039.8040928
TTGACGA1553.473542E-837.4195149
GTCTTGC20000.036.861571
GTCTCGC9650.035.5510981
TCGCTCT11000.034.928144
CTGTCGC13650.030.805999
CTCGCTC12100.028.7598233
TACCGTA1804.7032754E-628.1945657
CGCTCTG13200.026.9129925
GTGTAAC6750.025.9531441
GTCTTAC9050.025.0031991
GTATATA8800.024.884051
TAGTCCG1751.2563789E-424.8572485
GTCTCAC22350.024.4943681
GTACAGA11700.024.331071
TCTCGCT14900.023.8423842
GTACTAA4900.023.8345181
TTGCTCT29450.023.1384774
GTATAAT6850.022.3774811
GTGTAAA13100.022.2880041
Produced by FastQC (version 0.11.5)