FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2047527
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8500.052.8814168
GTCTCGC8250.042.2677761
CTGTCGC10900.041.902939
GTCTTGC18300.034.9344331
TCTCGCT10350.030.8410452
TCGCTCT10850.030.7525564
CTCGCTC11400.028.6325973
CGCTCTG11150.027.9584665
GTATTAT5650.027.0018161
CTATACG1100.0096747426.3631699
GTCTCAC20950.025.6607781
GTGCGTA1450.001212408225.0509431
GTGTTAG11100.024.8703981
GTATTAG7100.024.5569841
GTACGGA1500.001477188724.2159161
GTGTACG2101.531762E-524.2159141
GGAGTGC45150.023.9247975
GTGTTAC8200.023.9205971
GTCTTAC7950.023.7590081
TTGCTCT25750.023.66224
Produced by FastQC (version 0.11.5)