FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064574
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6000.048.3364948
CCGTACG657.336038E-444.618318
GTATACG1151.3885438E-744.2308121
GTCTCGC9600.041.6309361
TCGCTCT10350.038.544034
CTGTCGC9450.034.5269059
GTCTTGC15850.033.0086671
CGCTCTG11600.032.498195
CTCGCTC11850.031.8287643
TCTCGCT12300.030.6709862
GTATTAG7050.027.8291131
ACCGTGT7300.027.8100388
CTATACG1050.007693292627.6315654
GTATAAT6400.027.249341
GTGTTAG13800.025.2747521
GTATAGC4600.025.274751
TTCACCG7000.024.8557595
CCGTGTT8200.024.7583189
GGGTTTC33800.024.2932971
TAATACT5750.023.9673774
Produced by FastQC (version 0.11.5)