FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2064574
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTACG1956.537448E-933.697051
ACGAGCC5800.032.501217145
GTATTAC5450.032.151311
GTCTCGC8000.030.1167351
TCTGTCG8450.029.1712768
GTCTTGC16900.027.648861
GTATAAG5450.026.7927591
TAATACT6250.026.6771224
TCGCTCT9650.026.29254
GTACTAA4450.026.2508811
GTATAGG5300.024.795941
GTGTAAG10600.024.1071641
ATGGCGT9350.024.0371696
GGGTTTC33550.023.7201771
GAGTGCA46050.023.1430666
CTGTCGC10100.022.9700439
GTATAGT6050.022.9288441
GTGTTAC7050.022.7833461
GTATTAA5450.022.7738461
GGAGTGC42550.022.6607745
Produced by FastQC (version 0.11.5)