FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347200

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347200
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2038661
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC7500.037.9429861
TCGCTCT8750.033.139674
GTCTTGC17350.032.8037341
GTACTAT6100.031.1008071
GTGTTAC7550.029.9600731
CTCGCTC9600.027.9407083
TCTGTCG7800.027.883998
CCGTGTT6850.027.517639
GTACTAG4550.027.26251
TACTTCG1358.08667E-426.8512487
CGCTCTG9950.026.2299215
TTCACCG6850.025.4002655
GTATTAG7250.025.1611291
ACGACGA1450.001227041124.99944145
ACCGTGT8750.024.8565838
TCTCGCT11000.024.3870122
TGATTCG1801.5215267E-424.1655315
GTGTTAT7650.023.8455141
GTGTAAA11950.023.813591
GGGTACG1851.753521E-423.6650621
Produced by FastQC (version 0.11.5)