FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347203

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347203
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2253983
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAACGC1551.4612979E-632.7556533
GTCTTGC17600.031.7785911
GTCTCGC9300.030.4605831
GTCTTAC8100.027.7992611
TCGCTCT9400.027.0059954
GTATTAC5750.026.5282171
ACTGTCG2559.49949E-825.58768
GGCGTAC1450.00121351325.0471671
GTCGTAA1751.2404314E-424.9040371
GGTATAC3801.2732926E-1124.8494261
GTGTAAA13200.024.7625391
CCGTGCG2051.2914472E-524.7554849
GTGTACG2351.3099925E-624.7274131
CTGTCGC10900.024.6094829
AACCGTG2951.4302714E-824.5756637
TCTGTCG8600.024.4470678
TCTCGCT11200.023.9646532
ACTTACG1550.001809450923.3865178
GTACTAT5000.023.2437691
GTGTAAG10950.023.2172361
Produced by FastQC (version 0.11.5)