FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2253983
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7800.044.614558
GTCTCGC7800.040.2394751
TCGCTCT8700.037.4968034
GTACCGT1251.2084205E-535.036421
CTGTCGC11250.033.5104839
GTATTAA5250.031.9776841
GTACGGA1453.3345743E-530.203811
CTCGCTC9750.028.2540023
TAAACCG1306.4861996E-427.8840965
GTATTAT6050.027.749231
GTGTTAT5900.027.2175561
GTATAAT6250.026.8612541
GTGTAAA13900.026.7814351
TCTCGCT11650.026.757972
GTGTAAG10800.025.682561
GTGTAAC6050.025.3362541
GTATAGG4900.025.3239421
CGCTCTG10150.024.9995335
GTGTAAT7400.024.6596431
GTATTAC3952.1827873E-1124.0228611
Produced by FastQC (version 0.11.5)