FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2068469
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8400.039.9786951
CGCCGTT1951.70985E-1037.179394145
TCGCTCT8850.035.2224924
TCTGTCG8300.034.9379848
GTCTTGC16850.031.1948341
GTATTAA4500.030.8241561
CGCTCTG10400.028.5802785
CTGTCGC11600.027.4986029
GTGTTAG10700.027.2914311
GGTTACG1100.00935233126.5471171
TCTCGCT11500.026.4781952
CTCGCTC10750.026.3003523
CTGTACG1701.0325105E-425.5856974
GTACGGT1450.001227423324.9981233
GTATTGA5650.024.5502131
GTCTAGC7150.024.5050331
GTATAGT4201.8189894E-1224.3348581
GTATTGG5400.022.9829221
TGTACGA1600.00217657422.6550985
CCGACGT1952.616098E-422.307636145
Produced by FastQC (version 0.11.5)