FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2132013
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC4000.036.3333781
TCTGTCG8750.033.9697158
GTCTCGC9300.028.9104331
GTATTAG4850.028.467391
GTCTTGC19400.027.7182481
GTGTTAG10650.027.2926791
GTATAAC2405.1612005E-827.2500361
TCGCTCT10000.026.830574
CGGTACT1100.00966421326.3691084
CGTGTAC1450.001225415725.0051883
GTATTAT3901.8189894E-1124.2222541
CTAACGT1801.518933E-424.1716844
TCTCGCT11250.023.854992
CTTGTCG2202.2312075E-523.0670179
GCTATAC3152.8627255E-823.020653
GTGTAAT6650.022.94741
TTGCTCT25100.022.8234624
CTGTCGC13350.022.8078379
GTCTCAC22150.022.6365971
ACGAACT2003.1002788E-421.7545154
Produced by FastQC (version 0.11.5)