FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347213

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347213
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1899973
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG7500.054.1354148
CTGTCGC10700.040.655779
TCGCTCT8050.037.8404924
GTCTCGC7550.034.627371
GTCTTGC17900.034.079261
GTATAGT4400.031.3591921
TCTCGCT8550.029.6896652
GTGTAAA10350.028.7678551
CGCTCTG10200.028.4324655
TAGTACG1050.007695858427.6295664
CTCGCTC9400.026.2313163
TTGCTCT25050.025.768294
GTCTCAC21400.025.4513871
GTCTTAC7450.025.3443431
GTATAAG4600.025.2595791
GTGTAAC5250.024.898731
TAACGCT1751.253064E-424.8666084
GTGTTAG13100.024.3918821
GTGTAGT9700.023.9575391
CCCGTAT2151.8663279E-523.6055588
Produced by FastQC (version 0.11.5)