FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3095713
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG12650.043.558398
GTCTCGC12950.037.0272751
CTGTCGC17600.033.7792479
GTCTTGC26850.031.1172451
TCGCTCT14550.028.408274
TCTCGCT16300.027.1430972
GTATAAT6300.025.370541
GTCTCAC33650.025.2608761
CGCTCTG14950.025.2163855
CTCGCTC16000.024.9273223
TTGCTCT37700.023.6590184
GTGTTAG17950.023.070551
GAGTGCA75900.022.9254676
TTGTACG1952.6125935E-422.312574
GTTCTAC7850.022.2120761
GGAGTGC71500.022.2054065
GTGTATA12200.022.0338041
GTCTAGC11050.021.6969781
CTGGAGT86300.021.5110743
GGCTAGA17850.021.1647281
Produced by FastQC (version 0.11.5)