FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2241134
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8900.057.835538
GTCTCGC10050.041.1863021
CTGTCGC14250.035.1043249
TCGCTCT12050.034.9046754
TCTCGCT11700.034.716192
GTACTAT4300.033.775791
TACACCG1957.003109E-933.4598965
CTCGCTC12750.031.2820473
ACTATCG1703.014542E-629.8521447
GTCTTGC20400.029.545541
GTGCTAC7850.028.6771551
GTGTAAC6350.028.5897451
CGCTCTG13650.028.14885
TTACGCT1555.494395E-528.0712814
TACTACG1306.468745E-427.896942
GTATTAG6450.027.0206341
GTCTCAC25700.026.843211
TTGCTCT26750.025.753874
ATACGGT1701.0320398E-425.5875516
TGTTACG1450.00122376225.011052
Produced by FastQC (version 0.11.5)