FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347219

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347219
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1904969
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9500.043.574721
TCTGTCG7300.042.7058458
TCGCTCT9850.041.9623834
TCTCGCT10850.035.4252782
CTCGCTC11100.033.9704933
CGCTCTG10750.033.7194485
GTATCGG1102.4125431E-433.011151
GTATTAA3750.032.9231221
GTCTTGC17650.030.8602811
CTGTCGC11350.030.023079
GTACGGA1702.9735656E-629.9042171
GGATACG1504.349563E-529.049811
GTATACG1306.420368E-427.932511
TCTATCG1100.00967333726.3638618
GTGTTAC6900.026.3132341
GTGTAGA6700.026.0147551
GTGTAGG8950.024.3434181
GGAGTGC43450.023.5260075
TTCGCTC2552.7047008E-622.7494723
GGTATAC3655.5806595E-921.8868451
Produced by FastQC (version 0.11.5)