FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985599
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG8250.042.1822938
GTCTTGC16500.032.5795171
CTGTCGC11350.031.9386869
TCGCTCT9150.031.7047254
GTCTCGC8100.031.3891721
GTATAGG5250.027.6737171
CTCGCTC10050.027.4208413
TCTCGCT9700.026.921762
GTATTAG7400.026.5050621
GTGTAAC5850.026.0771581
TAATACT7300.025.8306664
GTCTTAC7600.025.8075641
TATACTG6500.024.5387385
GGCTAGA10600.023.3007491
GTACTCG1902.1650371E-422.9400561
CGTACAG1600.00217575722.6565065
GCATCGT1952.6158028E-422.3079456
GTATAAT5900.022.1624281
GTATAAG5900.022.1624281
CGCTCTG10250.021.9270765
Produced by FastQC (version 0.11.5)