FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347222

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347222
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1985599
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC8800.041.4714241
TCGCTCT9400.040.1027534
TCTGTCG7600.038.154448
GTTCGTA850.002743511534.115422
CGCTCTG10050.033.181085
GTACGAG1551.3908721E-632.9630931
TCGTGCG1102.4383047E-432.9515659
CTCGCTC10800.032.2201163
GTCTTGC15750.031.049591
GTACTAT4300.030.553831
TCTCGCT11400.030.5243232
GTATAGA5050.028.9068151
GTGTTAC7000.028.1531721
CTGTCGC11000.027.6793149
GTATTAG6900.025.3877241
GTATTAT6450.023.7640911
TACACTG11300.023.7367885
GTGTAAG8650.023.6267241
GTATAAT6200.023.5450671
GTATAAG5600.023.4609761
Produced by FastQC (version 0.11.5)