FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2880191
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC13100.042.349681
TCGCTCT14550.041.352744
TCTGTCG11700.039.038148
CTCGCTC16150.034.1149943
CGCTCTG15550.033.568855
TCTCGCT16950.032.507672
GTCTTGC24200.032.2757451
GTATTAT9200.030.1511341
CTGTCGC16700.029.5207149
ACGACGG7100.026.550459145
TAATACT8000.026.2782964
GCGTTAG2258.5895226E-725.954661
GTGTTAG18200.025.268361
GTCTCAC33250.025.247251
GGAGTGC64050.025.2417875
GTGTAGT14600.024.9991381
GTATAGC6150.024.9259681
GAGTGCA68750.024.7816146
GGCGTAC1500.001436582824.3324951
CCGTTCG1801.5213399E-424.1664669
Produced by FastQC (version 0.11.5)