FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347227

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347227
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2913870
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACGAC657.271696E-444.6987951
TCTGTCG10700.044.0403488
GTCTCGC12100.039.6193851
GTCTTGC26700.035.637661
TCGCTCT13250.033.9348564
CTGTCGC14750.033.914529
CGCTCTG14750.030.4733945
CTCGCTC14250.029.517253
TCTCGCT15600.028.8288142
GTATAGG7550.025.9756221
TACACCG2101.5568643E-524.165735
GTATTAG10000.023.9697281
GTATAGT7950.023.7550181
GTGTTAG19150.023.5164661
GTACTAG4950.023.4781511
TTGCTCT38750.023.3942184
TACGACC2202.2219141E-523.0799582
GTCTTAC10850.022.0919151
CTTGCTC37800.022.0632153
GTCTCAC34000.022.0042951
Produced by FastQC (version 0.11.5)