FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2913870
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC10100.038.3042561
TCTGTCG9900.035.1503458
TCGCTCT12250.034.9136124
GTCTTGC23600.033.0951461
GTATAAG6650.029.637031
CGATACT1504.4056535E-528.996054
CTGTCGC12800.028.8857579
GTATAAT9250.026.8305591
GTGTAAG13300.026.3440271
TCTCGCT15850.026.0734772
GTGTAAT11950.025.6551131
CTCGCTC15550.025.6413543
CGCTCTG15050.025.0489985
TTGCTCT35500.024.91214
GGAGTGC63100.024.5871375
GGTATAC5350.024.5590321
GAGTACG1801.4526403E-424.3316351
GAGTGCA68600.023.9897276
TATACTG6700.023.8051785
GTATTAT8000.023.7233431
Produced by FastQC (version 0.11.5)