FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2621678
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC9750.041.9220731
TCGCTCT11750.039.4859544
TCTGTCG11500.035.934428
CGCTCTG13550.033.7075085
TCTCGCT13550.031.5691572
CTCGCTC13700.030.6919523
GTATAGG7550.029.9691281
CTGTCGC14000.029.5175619
GTCTTGC23550.026.964211
GGTATAC5000.026.2761551
GTATTAT9000.024.3297751
GTGTAAC8050.023.5741921
GTGTTAG18950.023.4952451
GGAGTGC55450.023.4032885
GTATAGT6550.023.4011571
GTACTAT5700.023.0492611
GTATAGA7000.022.93951
GTATAAC5100.022.8986111
GTCTCAC29000.022.651861
GAGTGCA60600.022.6111586
Produced by FastQC (version 0.11.5)