FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3213499
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG10900.037.912558
GTATTAA8550.031.434681
GTCTCGC13350.030.4705411
CTGTCGC15950.027.2725169
GTACGAT2456.328628E-826.6839331
GTCTTGC26750.026.6118891
TCGCTCT16050.025.3059944
GTATTAG10100.025.1721361
GTATAGT10000.024.6974621
GTCTTAC12200.024.4116671
GTATAGG6900.024.21321
GTGTAAT12650.023.5432681
GGTATAC5300.023.2994921
TCTCGCT16650.023.0911962
CGCTCTG16850.022.8029125
GTATAGA8000.022.6998751
GTGTAAG15050.022.6847881
GTGTTAC12550.022.5732611
GTCTCAC33950.022.4658551
GTGTTAG22000.022.4522381
Produced by FastQC (version 0.11.5)