FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347232

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347232
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3213499
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTCGC11850.036.951661
TCTGTCG11700.035.9403088
TCGCTCT14000.030.5498834
GTCTTGC27300.029.940321
CTGTCGC15450.028.6247029
GTGTAAC8700.027.6818891
TCTCGCT15700.025.8599722
TAATACT9350.024.809844
GTAATAC8200.024.7542633
CTCGCTC15600.024.6295853
GCGTTAG2401.5106943E-624.3265111
GTATTAA7700.022.7468661
GTCCGTA1952.5023383E-422.4552381
CGCTCTG15700.022.1653425
GTCTAAT8500.021.4645671
TTGCTCT37200.021.4355834
GTACTCG3055.347574E-721.3935246
GTATATA12000.021.2856961
GTATAGT7550.021.2655581
GTATTCG2104.1291412E-420.8512941
Produced by FastQC (version 0.11.5)