FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2381875
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGCTC7850.020.5141073
TCGCTCT7700.020.4586564
GTCTCGC7200.020.4220581
AGTACCG1205.7103032E-520.4216275
GTCTTGC14600.020.3820921
TCTGTCG6250.020.1598178
TCTCGCT7700.020.004442
CGTGTTA2653.6379788E-1219.81072610
TTACGCT900.00527236519.448354
TGTCGCC8150.018.46570410
GTATAGG3250.018.3125251
GTCGTAG1000.00967861617.504621
GTCGCCC7250.017.37878811
CTGTCGC8400.016.6661689
AATATCG1906.1278333E-616.57879670
TACGCCT2351.5413207E-716.386274
TTGCTCT18150.016.0087284
GTATAGT3301.2551027E-1015.9132911
CGCTCTG8650.015.7841815
GTATTGG4700.015.64242651
Produced by FastQC (version 0.11.5)