FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347458

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347458
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2381875
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTTA2250.029.55596470
GTAGCGC1101.01755E-625.4548976
GTCTTGC14300.022.0329021
GTCTCGC6200.020.3271271
TCTGTCG6250.019.6002718
CTGTCGC6650.018.4213079
TCGCTCT7350.018.0951084
GTATTAC3650.017.2641371
TGTCGCC7300.017.26051110
GTCTCAC16500.017.1856631
GTATAGG3501.8189894E-1217.0038031
CGTGTGT21800.016.6974870
GTGTAAA8600.016.6897811
GTATAGC3651.8189894E-1216.305021
GTCTTAC6500.016.1574611
CGTGTTA2602.7679562E-816.1540710
TCGCCCA7100.015.77486512
GTACTAG2704.39195E-815.5590371
ACTGTCG1350.002947575415.5557718
CACGTGT34900.015.4443470
Produced by FastQC (version 0.11.5)