FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492575
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG6700.031.8647048
GTCTCGC7900.027.9172651
CTGTCGC8500.024.2938679
TGTCGCC8100.023.76518610
TCGCTCT9150.023.7208354
TCTCGCT9350.022.4646172
TCGCCCA8500.021.82286512
GTCGCCC7950.021.5716711
GTCTTGC14850.021.4523241
CGCTCTG9600.020.4209615
GTATAAG5350.019.6302991
CTCGCTC10450.019.4299243
GTATAGG3900.018.8501191
CAACGCG950.00721250418.42123245
GTATTAC3500.018.0037861
TTACACT8500.017.7096084
TAGTCCG1000.00968172217.5036815
GTATTAG5900.017.207011
GTCTTAC5900.017.207011
CCGTTCG1653.3985645E-516.9695229
Produced by FastQC (version 0.11.5)