FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347463

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347463
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865179
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATACG500.007204724528.0053943
GGTCCGT1154.8989023E-827.3965826
GTATAAC2500.026.6051251
CGGTACT750.001831403223.3378284
GTCTCGC5200.022.8890271
TCTCGCT5200.022.215822
GATATCG1755.2586984E-922.0001170
TCGCTCT4950.021.9234164
GTCTTGC12150.021.0328581
CGATATA850.003791527120.58833770
TGCGTTA850.00379270520.58723412
TCTGTCG4850.020.2052028
TAGCCGT1055.461218E-420.0038554
TTAACGG900.0052779219.44454235
GTATAGT3650.019.1817781
ACTGTCG1107.4819557E-419.089988
TGTCGCC6100.018.93350410
CGATATG1301.058045E-418.84624970
GTCTTAC3850.018.1853221
GTATAGA3900.017.9521751
Produced by FastQC (version 0.11.5)