FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865179
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGTCG5800.024.740838
CTGTCGC6600.024.3933989
TGTCGCC6450.023.33281510
GTCTCGC6000.022.7531551
GTCTTGC11850.022.154971
GTACTAC1807.2650437E-921.3918551
TCGCCCA6800.020.58777812
GTCGCCC6650.020.52585811
CTATACT2950.020.1695844
GTATATA4200.020.0027731
TATACTG3500.019.9995575
GCGTAAT900.00527875119.44401411
TGCGTAA1107.480625E-419.09048510
GTATAGG2405.020411E-1018.9609621
TCTCGCT7200.018.9599462
GTCTTAC5800.017.5024261
GTATTAA3053.8198777E-1117.2155021
CCGTGTT3053.8198777E-1117.2127329
GGTATAC2451.3274985E-817.1452351
GTGTTAG6600.016.972051
Produced by FastQC (version 0.11.5)