FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005347469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005347469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1718768
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACTGATATATCTCGTATGCCGT25330.14737300205728754No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG6750.029.0371770
AAGGGGG9500.020.2632569
GTATAAG2653.6379788E-1219.8137191
GTATAGA2302.8376235E-1019.7850021
TATTAAC1801.701901E-719.4467962
GTATTAC1852.270699E-718.921211
GTATTAA1551.9834673E-518.0667021
ATATTAG1752.7945898E-618.0021761
GTATATA1803.6563433E-617.5021171
GTATAAA2401.0290023E-817.5021171
AAAAGGG11150.017.26465267
GGTATAC1653.393749E-516.971751
AAAGGGG11450.016.50662668
AAAAAGG12100.016.48767966
CGTATGC12250.016.28673641
GGCTAGA4950.016.2645931
TCGTATG12300.016.22100340
TATGCCG12600.015.83294643
GTATCAA2009.957861E-615.7519051
GTATAAC1350.00294502515.5574371
Produced by FastQC (version 0.11.5)